There is a little bit of trickery involved I have to admit, I hadn't written a word on 01.12, just thought very hard about not wanting to write but feeling bad about the mounting pressure to do.

It'd probably be best to do a short review on what I did in over july, I have restarted my labwork in second week of november, a lot was attempted before, but I wasn't making good notes about what I was doing, luckily (?) I failed at what I set out to do then and data I obtained there was of extremely poor quality and not statistically signifiant because I was preforming in-vitro cultures very poorly, I have to retry doing almost all experiments I did then.

Asceptic cultures in petri plates.

DNA extraction from some of them with a kit

PCR validation of mutants with ready made primers

Collecting the H. schatti cysts from old plant material, sterilizing cysts

Germinating the cysts in custom hatching contraptions

Infecting in vitro cultures

Letting the the new population of the nematode reset (i think) it's epigenome on mustard.

Counting the changes under the microscope (truly miserable experience, I will try my best to automate it somewhat)

Collecting non-infected plant material for cDNA synthesis and expression analisis

The funny bit about what I did is that I either lost, or hadn't seeded them (p. sure the second one, I was exhausted and got confused) a huge part of seedlings needed for the experiment, and from the small amount I had like 20% got infected because the culture has to be done antibiotic free because the nematode has to develop in natural-ish ciurcumstances.
Also it's very tragicomic how I am always 20 minutes late, it's some serious mental ilness I have, I have decided to work on it and squash that bad habit too.