Season's Greetings

I am grossly irrensponsible, I procrastinate on serious resposibilities to an obscene degree way beyond any notion of decency, unmatched by anyone I know.
I have 60 days to write my 40-60page undergraduate thesis, to fit the theme of the jolly season I decided to motivate myself to start working on this very important matter by creating a advent callendar of sorts, my hope is that I will have finished the early draft of the dissertation by Christmas.

It felt disgusting typing out what I wrote so far, the weight of my unwarranted optimism crushes my soul, it's a shameful thing to start that late on something that important.
Nevertheless time only moves forward, and I always enjoyed the nature of this internet emotional exhibitionism.

Topic and Motivation

mlo is code for a family of genes related to calcium signalling in higher plants, mlo11 is one of those genes, or so I've been told, it triggers some innate immune response when it comes to infection of plants by plant infecting fungi.
FACTCHECK: FALSE mlo4,6,12 expression lets a mildew (mildew locus O) fungi infect the plant according to Flor's gene-for-gene model of plant-pathogen interactions
FACTCHECK: FALSE² not Flor's R gene, an S gene, in a loss-of-susceptibility model, mlo2,6,12 when broken confer resistance, mlo has a silencing effect on the plant immune system, some evidence exists that it is involved in facilitating symbiosis between plants and fungi,
and mlo11 is some other thing related to touch response of root developement, but all MLO protein are influenced by calcium signalling, there is a lot of nuance there that I am unaware of (yet?).
It also is suspected to influence the process of infection and immune response under challenge from a nasty sugar beet parasite, Heterodera schachtii which also can succesfully infect a classic model plant Arabidopsis thaliana, a weed that grows fast and is well understood due to being sequenced early and being used for funky-looking mutant hunting in the days before the human genome project.

My motivation as someone enamored by molecular biology was to do some practical work in it. The prof I work under on that dissertation was one of the few available to work on something distinctly molecular biology related.
The goal is to confirm if the mutation breaking the gene leads to a phenotype (less/more infection) and if it's expressed and how much in various plant tisuses (cDNA rt-qPCR) comparing wild type and four tDNA instertion mutants bought online. The job in the lab is to create in vitro A. thaliana cultures for confirming that, counting changes related to infection and do the molbio labwork with few different PCR/gel based tools.